Ethylene-mediated root endodermal barrier development in impeding Cd radial transport and accumulation in rice (Oryza...

Ethylene plays crucial roles in the adaptation to cadmium (Cd) stress. Nevertheless, the impact of endogenous ethylene on radial transport of Cd in different rice cultivars are insufficiently understood. Herein, we investigated how ethylene involved in the formation of endodermal barriers in roots of Nipponbare with low-Cd accumulation and IR32307 with high-Cd accumulation ability and further assessed its influence on Cd radial transport. Our analysis indicated that both Cd stress and external ACC (1-aminocyclopropane-1-carboxylic acid, ethylene biosynthesis precursor) promoted the ethylene production. Intriguingly, the positive response of ethylene signal to Cd was more intensive in roots of Nipponbare than that of IR32307. The increased endogenous ethylene in rice roots promoted development of casparian strips (CSs) and suberin lamellae (SL). Specifically, external addition of ACC decreased the percentage of the D_TIP-CS/D_TIP-SL to root length by 44.4-79.6%/49.3-11.4% in Nipponbare and 18.7-19.9%/10.7-35.3% in IR32307, individually. The intrinsic molecular mechanism was mainly due to changes in the genes expression levels related to CSs/SL biosynthesis. Simultaneously, the analyses of apoplastic tracer (Propidium Iodide, PI) and cell-to-cell tracer (Fluorescein Diacetate, FDA) confirmed that the ethylene-mediated endodermal barriers were functional, which were in accordance with the increased/reduced Cd transport in roots. Eventually, the results of transcriptome analysis further shed a comprehensive insight that ethylene constructed the endodermal barrier through phenylpropanoid and cutin, suberine and wax biosynthesis to reduce Cd radial transport in rice, which are beneficial for the breeding of rice with low-Cd accumulating capacity in the future.