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E2F1 and E2F7 regulate gastric cancer cell proliferation, respectively, through transcriptional activation and

Tianyi Wu1, Fengli Jiang2, Fan Wu1

  • 1Department of Pathophysiology, College of Basic Medical Sciences, China Medical University, Shenyang, China.

The Journal of Biological Chemistry
|November 29, 2024
PubMed

Insights

This study reveals E2F1, E2F7, and MYBL2 are upregulated in gastric cancer (GC), impacting patient prognosis. Targeting these factors or their balance offers potential new therapies for GC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Gastric cancer (GC) is a prevalent digestive tract malignancy with poorly understood molecular drivers.
  • Identifying key molecular players is crucial for developing effective targeted therapies.

Purpose of the Study:

  • To investigate the roles of E2F1, E2F7, and MYBL2 in gastric cancer pathogenesis.
  • To elucidate the regulatory mechanisms and therapeutic potential of targeting these factors in GC.

Main Methods:

  • Bioinformatic analysis of GC tissues.
  • Gene expression analysis and correlation with patient prognosis.
  • In vitro and ex vivo cell culture experiments, including gene knockdown and chromatin immunoprecipitation assays.
  • Analysis of the PI3K/AKT signaling pathway and E2F7 nucleocytoplasmic distribution.

Main Results:

  • E2F1, E2F7, and MYBL2 were found to be upregulated in GC tissues, correlating with poorer patient outcomes.
  • E2F1 and MYBL2 knockdown inhibited GC cell proliferation and induced apoptosis, while E2F7 knockdown promoted proliferation.
  • MYBL2 acts as a direct transcriptional target of both E2F1 (activation) and E2F7 (repression), mediating their effects on cell proliferation via the PI3K/AKT pathway.
  • Differential nucleocytoplasmic distribution of E2F7 was observed in GC cells.

Conclusions:

  • MYBL2 is a key downstream effector of E2F1 and E2F7 in regulating gastric cancer cell proliferation.
  • Targeting E2F1, E2F7, MYBL2, their expression balance, or E2F7 localization presents novel therapeutic strategies for gastric cancer.
Keywords:
E2F transcription factorMYBL2cell proliferationgastric cancernucleocytoplasmic distributiontranscription regulationtumor cell biology

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