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Sucrase and cellular development.

N Kretchmer, J S Latimer, F Raul

    Ciba Foundation Symposium
    |January 16, 1979
    PubMed
    Summary
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    Intestinal sucrase-isomaltase enzyme activity changes during cell differentiation. Antibodies reveal enzyme location, suggesting subunits aggregate into the brush border enzyme complex.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Enzymology

    Background:

    • Intestinal cells undergo significant morphological and biochemical changes from crypt to villus.
    • Enzymic activities serve as markers for these cellular differentiation processes.
    • Sucrase-isomaltase is a key enzyme complex indicative of differentiated intestinal cells.

    Purpose of the Study:

    • To investigate the characteristics and localization of the sucrase-isomaltase enzyme complex in intestinal cells.
    • To explore the relationship between enzyme subunits and the functional enzyme complex.
    • To examine sucrase-isomaltase activity in the California sea lion to understand gene complex differentiation.

    Main Methods:

    • Utilizing enzyme activity assays to track cellular changes.

    Related Experiment Videos

  • Generating specific antibodies against sucrase-isomaltase for antigen localization.
  • Determining the molecular weight of enzyme components.
  • Comparing enzyme activity in different species (California sea lion).

    • Main Results:

    • Sucrase-isomaltase is inducible by steroids and protected by sucrose.
    • Antibodies localized enzymatically active and inactive antigens in crypt and villus cells, respectively.
    • Higher molecular weight enzyme complexes reside in the brush border, while lower molecular weight subunits are in the cytosol.
    • California sea lions exhibit isomaltase activity but lack sucrase activity.

    Conclusions:

    • Sucrase-isomaltase exists as aggregated subunits in the brush border, with smaller, antigenically active subunits in the cytosol.
    • The distinct sucrase and isomaltase activities in the California sea lion support separate gene complexes for each enzyme.