Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cerebellar microRNA-206 tunes Purkinje neuron firing dynamics to control sensorimotor gating.

bioRxiv : the preprint server for biology·2026
Same author

Role of striatal dopamine signaling in compulsive eating associated with obesity.

Current opinion in behavioral sciences·2026
Same author

Review: "The Disappointment Dilemma: Short- and Long-Term Learning From Negative Outcomes".

The European journal of neuroscience·2026
Same author

Correction: Vitamin A deficiency induces sex-specific reward processing alterations through a dysregulation of the mesolimbic dopamine transmission in mice.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology·2026
Same author

Vitamin A deficiency induces sex-specific reward processing alterations through a dysregulation of the mesolimbic dopamine transmission in mice.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology·2026
Same author

Artificial intelligence-driven whole-brain cell mapping with highly multiplexed in situ hybridization.

Neuron·2026

Related Experiment Video

Updated: Jun 6, 2025

Intact Histological Characterization of Brain-implanted Microdevices and Surrounding Tissue
11:31

Intact Histological Characterization of Brain-implanted Microdevices and Surrounding Tissue

Published on: February 11, 2013

16.8K

Precise 3D Localization of Intracerebral Implants Using a Simple Brain Clearing Method.

Julien Catanese1, Tatsuya C Murakami1, Adam Catto2

  • 1Laboratory of Molecular Biology, The Rockefeller University, New York, NY 10065, USA.

Journal of Integrative Neuroscience
|November 29, 2024
PubMed
Summary

This study presents a new method for precisely locating brain implants in mice using advanced tissue clearing and microscopy. This technique improves accuracy for functional studies by enabling virtual brain slicing.

Keywords:
CUBICbrainclearingelectrodelight sheet microscopymethodsilicon probe

More Related Videos

A Micro-CT-based Method for Characterizing Lesions and Locating Electrodes in Small Animal Brains
05:12

A Micro-CT-based Method for Characterizing Lesions and Locating Electrodes in Small Animal Brains

Published on: November 8, 2018

8.4K
Investigation of Spatial Interaction Between Astrocytes and Neurons in Cleared Brains
05:17

Investigation of Spatial Interaction Between Astrocytes and Neurons in Cleared Brains

Published on: March 31, 2022

2.5K

Related Experiment Videos

Last Updated: Jun 6, 2025

Intact Histological Characterization of Brain-implanted Microdevices and Surrounding Tissue
11:31

Intact Histological Characterization of Brain-implanted Microdevices and Surrounding Tissue

Published on: February 11, 2013

16.8K
A Micro-CT-based Method for Characterizing Lesions and Locating Electrodes in Small Animal Brains
05:12

A Micro-CT-based Method for Characterizing Lesions and Locating Electrodes in Small Animal Brains

Published on: November 8, 2018

8.4K
Investigation of Spatial Interaction Between Astrocytes and Neurons in Cleared Brains
05:17

Investigation of Spatial Interaction Between Astrocytes and Neurons in Cleared Brains

Published on: March 31, 2022

2.5K

Area of Science:

  • Neuroscience
  • Biomedical Engineering
  • Microscopy

Background:

  • Accurate localization of intracerebral implants is crucial for rodent brain studies, especially for deep brain nuclei.
  • Traditional histological methods are prone to errors and complicate data interpretation.

Purpose of the Study:

  • To introduce a novel, precise method for localizing intracerebral implants in rodent brains.
  • To overcome limitations of traditional histological techniques in brain implant studies.

Main Methods:

  • Utilized tissue-clearing techniques and light-sheet fluorescence microscopy.
  • Employed a simplified Clear, Unobstructed Brain/Body Imaging Cocktails and Computational Analysis (CUBIC) method.
  • Demonstrated the technique in ChAT-ChR2-EYFP transgenic mice with optrode probes implanted in the midbrain interpeduncular nucleus (IPN).

Main Results:

  • Precisely visualized electrode localization in the IPN using DiD dye on the electrode surface.
  • Generated 3D brain videos from multiple orientations to showcase the method's potential.
  • Validated electrode placement through recorded optogenetic responses in the IPN.

Conclusions:

  • The developed method enables precise localization of brain implantation sites in transgenic mice with cell-specific fluorescence markers.
  • Facilitates virtual brain slicing in any orientation, enhancing its utility for functional studies in mice.