Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spindle Assembly02:50

Spindle Assembly

3.6K
Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
3.6K
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

5.5K
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
5.5K
Meiosis vs. Mitosis02:57

Meiosis vs. Mitosis

52.9K
Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
52.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immunosuppressive myeloid cells induce mesenchymal-like breast cancer stem cells by a membrane-bound TGF-β1-dependent mechanism.

Cell reports·2026
Same author

Stearoyl-CoA Desaturase-1 Drives Tumor Growth by Interacting With Histone Deacetylase-2 and Deacetylating Nucleophosmin-1.

MedComm·2026
Same author

Wave-optics simulation model for full-field modulation-based tensor tomography using a random wavefront modulator.

Optics express·2026
Same author

STING dampens the unfolded protein response to enable the presentation of self-antigens on MHC-I during inflammation.

Cell reports·2026
Same author

Barrier-to-autointegration factor protects against the cGAS-STING response to chromatin bridges.

PLoS genetics·2026
Same author

LRRK2 mutations block NCOA4 trafficking upon iron overload leading to ferroptotic death.

Journal of cell science·2026

Related Experiment Video

Updated: Jun 6, 2025

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos
13:59

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos

Published on: June 14, 2012

11.4K

Studying Mitotic Phosphorylation in Drosophila.

Éric Bonneil1, Myreille Larouche1, Virginie Emond-Fraser1,2

  • 1Institute for Research in Immunology and Cancer, Université de Montréal, Montréal, QC, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|November 29, 2024
PubMed
Summary

This study presents a phosphoproteomic protocol in Drosophila to identify key phosphorylation sites regulating mitosis. The methods allow for dissecting the roles of these sites in protein interactions and localization during cell division.

Keywords:
BiochemistryCell biologyDrosophilaMicroscopyMitosisPhosphorylationProteomics

More Related Videos

Microinjection Techniques for Studying Mitosis in the Drosophila melanogaster Syncytial Embryo
09:25

Microinjection Techniques for Studying Mitosis in the Drosophila melanogaster Syncytial Embryo

Published on: September 15, 2009

14.2K
Studying Mitochondrial Structure and Function in Drosophila Ovaries
09:53

Studying Mitochondrial Structure and Function in Drosophila Ovaries

Published on: January 4, 2017

23.9K

Related Experiment Videos

Last Updated: Jun 6, 2025

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos
13:59

Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos

Published on: June 14, 2012

11.4K
Microinjection Techniques for Studying Mitosis in the Drosophila melanogaster Syncytial Embryo
09:25

Microinjection Techniques for Studying Mitosis in the Drosophila melanogaster Syncytial Embryo

Published on: September 15, 2009

14.2K
Studying Mitochondrial Structure and Function in Drosophila Ovaries
09:53

Studying Mitochondrial Structure and Function in Drosophila Ovaries

Published on: January 4, 2017

23.9K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Mitosis regulation relies on reversible protein phosphorylation.
  • Drosophila is a powerful model for studying conserved mitotic mechanisms.
  • Understanding phosphorylation dynamics is crucial for cell division research.

Purpose of the Study:

  • To present a phosphoproteomic protocol for identifying mitosis-specific phosphorylation sites in Drosophila.
  • To provide methods for dissecting the functional roles of these phosphorylation sites.
  • To highlight the advantages of using Drosophila for these studies.

Main Methods:

  • Phosphoproteomic analysis of Drosophila cell cultures.
  • Identification of phosphorylation sites dependent on mitotic kinases and phosphatases.
  • Functional dissection using Drosophila embryos to study protein interactions and localization.

Main Results:

  • A detailed protocol for phosphoproteomic analysis in Drosophila is established.
  • Identification of novel phosphorylation sites critical for mitotic regulation.
  • Demonstration of methods to link identified sites to specific protein functions during mitosis.

Conclusions:

  • The developed phosphoproteomic protocol in Drosophila is effective for identifying key regulatory phosphorylation sites in mitosis.
  • The methods enable functional characterization of these sites in vivo.
  • Drosophila offers a robust and advantageous system for dissecting mitotic phosphorylation events.