Related Experiment Video
Updated: Jul 6, 2026

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
Published on: July 31, 2012
Production of functional bovine IL-22 in a mammalian episomal expression system
Laura I Klepp1, María Mercedes Bigi2, Luciana Villafañe1
1Instituto de Agrobiotecnología y Biología Molecular (IABIMO), UEDD INTA-CONICET, Argentina; Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria (Institute of Biotechnology, National Institute of Agricultural Technology, Argentina), Argentina.
Abstract:
Interleukin 22 is a member of the interleukin-10 superfamily of cytokines. This protein has a dual role as an inflammatory and anti-inflammatory molecule dependent on the context. IL-22 is produced mainly by immune cells and seems to have non-hematopoietic cells as its target. In this work, we report the production of bovine IL-22 for the first time in a semi-stable expression system in mammalian cells. We showed that this recombinant IL-22 possesses biological activity in bovine macrophages infected with Mycobacterium bovis and is easy to produce in large quantities. Given its role in the defence against infections, the IL-22 produced in this work has potential applications in scientific research as well as in immunotherapy to treat diseases in cattle.

