miR-317 regulates the proliferation and apoptosis of duck follicle granulosa cells by targeting VIPR1
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View abstract on PubMed
Summary
This summary is machine-generated.MicroRNA-317 (miR-317) influences duck follicle granulosa cell proliferation and apoptosis. miR-317 overexpression promotes proliferation, while its inhibition increases apoptosis and negatively regulates VIPR1 expression, offering insights into follicular atresia.
Area Of Science
- Reproductive Biology
- Molecular Endocrinology
- Avian Physiology
Background
- Vasoactive intestinal peptide receptor 1 (VIPR1) binds VIP, a prolactin (PRL) release factor, influencing PRL secretion and bird nesting behavior.
- Understanding the role of microRNAs in regulating avian reproductive processes, such as follicular atresia, is crucial for agricultural applications.
Purpose Of The Study
- To investigate the regulatory effects of miR-317 on VIPR1 gene and protein expression in duck follicle granulosa cells.
- To elucidate the impact of miR-317 modulation on cell proliferation and apoptosis in duck follicular cells.
Main Methods
- Histological analysis (HE staining) of Muscovy duck ovaries during nesting and laying periods.
- Isolation and identification (immunofluorescence for FSHR and LHR) of primary follicle granulosa cells.
- Transfection with miR-317 mimics or inhibitors, followed by EdU staining (proliferation), Annexin-V/TUNEL assays (apoptosis), qRT-PCR, and Western blotting (VIPR1 expression).
Main Results
- miR-317 overexpression significantly increased granulosa cell proliferation and decreased VIPR1 expression at both gene and protein levels.
- miR-317 inhibition significantly promoted granulosa cell apoptosis and increased VIPR1 expression.
- Histological analysis showed distinct nuclear characteristics in granulosa cells during brooding versus laying periods.
Conclusions
- miR-317 plays a dual role: promoting proliferation and inhibiting VIPR1 expression when overexpressed, while promoting apoptosis and upregulating VIPR1 when inhibited in duck follicle granulosa cells.
- These findings reveal a molecular mechanism underlying follicular atresia in ducks and provide a potential target for managing broodiness in Muscovy ducks.
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