Bisphenol A impairs oocyte maturation by dysfunction of cumulus cells

Bisphenol A (BPA) is a well-known environmental endocrine disruptor that has detrimental effects on reproduction. This study aimed to investigate whether BPA exposure could disrupt the function of cumulus cells and influence oocyte maturation and development. Porcine oocytes at the germinal vesicle stage were exposed to BPA for 44 h. The results revealed that BPA exposure led to dysfunction in cumulus cells by inhibiting meiotic division, inducing endoplasmic reticulum stress, and disrupting steroid synthesis. Furthermore, BPA exposure significantly increased reactive oxygen species and caused abnormal distribution of mitochondria in the oocytes. Notably, matured oocytes in the MII stage from the BPA-exposed groups showed significantly reduced development to the blastocyst stage, along with increased autophagy and apoptosis. These findings suggest that cumulus-oocyte complexes are sensitive to BPA exposure during the germinal vesicle stage, and the toxic effects of BPA on cumulus cells can severely inhibit oocyte and parthenogenetic embryos development.