Chronic murine schistosomiasis causes aberrant hemostasis.

Area of Science:

• Hematology
• Parasitology
• Immunology

Background:

• Schistosomiasis affects over 250 million people globally, causing significant morbidity and mortality.
• Schistosoma mansoni infection leads to intestinal pathology and liver damage.
• The impact of adult schistosomes on host hemostasis remains poorly understood.

Purpose of the Study:

• To investigate the effects of chronic Schistosoma mansoni infection on platelet function and overall hemostasis in a mouse model.
• To identify specific changes in platelet phenotype and coagulation parameters during infection.

Main Methods:

• A chronic mouse model of schistosomiasis was established.
• Platelet number, phenotype, and function were assessed using flow cytometry and proteomics.
• Whole blood aggregometry and longitudinal coagulometry were employed to evaluate hemostatic function.

Main Results:

• Schistosome-infected mice showed spontaneous whole blood aggregation without added stimulants.
• Platelets did not exhibit increased surface P-selectin or activated αIIbβ3.
• Proteomic analysis revealed increased MHC-I, IgM, IgG, and complement components on platelets.
• Prothrombin time and activated partial thromboplastin time were prolonged in chronic infection.

Conclusions:

• Chronic Schistosoma mansoni infection induces significant alterations in hemostatic function in mice.
• These changes include spontaneous platelet aggregation and impaired coagulation.
• The findings highlight potential clinical implications for bleeding disorders in schistosomiasis-endemic populations.