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Updated: Jun 6, 2025
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Genetic insights into the gut microbiota-duodenal diseases interplay: A Mendelian randomization and Bayesian
Xuehui Chen1, Hong Chang2, Chuanmei Gao3
1The Second Affiliated Hospital, School of Ocean and Tropical Medicine, Guangdong Medical University, Zhanjiang, China.
Background:
Many observational studies have shown a close association between gut microbiota and the risk of various duodenal diseases. Therefore, we urgently explore the potential causal relationship between gut microbiota and some duodenal diseases, mainly including duodenal ulcers and duodenitis.
Methods:
We conducted Mendelian randomization (MR) studies using genetic instrumental variables for gut microbiota from GWAS and duodenal disease datasets. Causal relationships were examined using multiple MR methods with Bonferroni correction. Bayesian Weighted Mendelian Randomization (BWMR) assessed causal relationships, employing ELBO and weighted data. Reverse MR analysis was conducted on microbiota showing significant causal relationships with duodenal diseases.
Results:
Through MR analysis, we identified three gut microbiota that promote the occurrence of duodenal ulcers (family. Coriobacteriaceae: OR = 1.003; 95 % CI = 1.0005-1.0056; p = 0.016, genus.RuminococcaceaeUCG003: OR = 1.006; 95 % CI = 1.002-1.007; p = 0.002, order. Coriobacteriales: OR = 1.003; 95 % CI = 1.0005-1.0056; p = 0.016), one microbiota that inhibits the occurrence of duodenitis (family. Acidaminococcaceae: OR = 0.994; 95 % CI = 0.988-0.999; p = 0.046), and one microbiota that promotes the occurrence of duodenitis (genus.Eubacteriumcoprostanoligenesgroup: OR = 1.006; 95 % CI = 1.0005-1.013; p = 0.033). Further confirmation of the occurrence of duodenal ulcers and the production of family.Coriobacteriaceae and order.Coriobacteriales microbiota was obtained through reverse MR analysis, indicating that the occurrence of duodenal ulcers also promotes the growth of these microbiota.
Conclusion:
Our study employs Mendelian randomization techniques to demonstrate a causal relationship between specific gut microbiota and duodenal ulcers and duodenitis. Additionally, our analysis suggests that duodenal ulcer occurrence promotes the growth of certain microbiota, emphasizing the intricate interplay between gut microbiota composition and these diseases.
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