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Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems
Published on: April 23, 2012
Replication kinetics, morphogenesis and interaction of shrimp parvovirus Penaeus stylirostris penstyldensovirus
D V Dominic Diya1, A Muneer1, Eldho Linu1
1National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Kochi, India.
Abstract:
Penaeus stylirostris penstyldensovirus (PstDV1) is one of the significant shrimp parvovirus which causes runt deformity syndrome in shrimps. In the current study, we attempted to elucidate the replication cycle of the virus in PmLyO-Sf9 cells. PstDV1 needs 4-5 h to complete replication in the cell line and release. Viral capsid gene expression leads to three peaks during the time course of the study at 4hpi, 12hpi and 72hpi. Immediate release of virus from the cell and reinfection in the cells observed. Calveloe mediated entry of virus to cells was noted through endosomal inhibition assay. Electron micrographs supported the findings which also depicted the receptor mediated entry, endosomal transport, viroplasm showing active virus replication and assembly of virus inside the nucleus to be released through cell lysis and occlusion bodies. Also, changes in cell cycle to make the cells to enter S phase was observed in cell cycle related genes and flow cytometric analysis. This reveal the potency of the cell line to study host-pathogen interaction, viral morphogenesis which could promotes successful development of antiviral formulations against the virus while preserving the environment for future generations.
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