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Live Cell Imaging of Chromosome Segregation During Mitosis
Published on: March 14, 2018
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Assessment of Chromosome Oscillations in Mammalian Cells by Live Cell Imaging
1Department of Molecular Oncology, Institute of Development, Aging and Cancer (IDAC), Tohoku University, Sendai, Miyagi, Japan. kenji.iemura.a6@tohoku.ac.jp.
Methods in Molecular Biology (Clifton, N.J.)
|December 1, 2024
Abstract:
In metaphase, chromosomes undergo a back-and-forth movement between two spindle poles called chromosome oscillation. This dynamic is necessary to maintain the robustness of chromosome segregation. This chapter describes the materials and methods required to observe chromosome oscillation in mammalian cell lines and calculate the deviation of amplitude (DAP), which is used to quantify the degree of chromosome oscillation.
