Related Experiment Video For Acentrosomal spindle
Updated: Jun 6, 2025

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
Published on: September 20, 2019
A Method for Analyzing Acentrosomal Mitotic Spindles in Human Cells
Shotaro Okuda1, Takumi Chinen2, Daiju Kitagawa1
1Laboratory of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
Abstract:
Centrosomes, the major microtubule organizing centers, facilitate mitotic spindle formation. However, recent studies have revealed that some cancer cells lack centrosomes. These findings suggest that certain types of cancer cells drive centrosome-independent mechanisms for the assembly of mitotic spindles. Therefore, analyzing the systems of acentrosomal spindle formation is beneficial for understanding the divergence of mitotic processes in cancer cells. Here, we focus on a method for generating acentrosomal cells using the PLK4 inhibitor centrinone and describe the proper conditions, spindle pole staining markers, and microscopy settings for observing acentrosomal spindle formation in human cells.
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