Application of selenium isotopes to define selenium bioreduction in coal waste rock: Elk Valley, British Columbia

Anthropogenic sources of selenium (Se), including coal mining, can release Se to the environment and raise Se concentrations in receiving waters above drinking water and aquatic limits. Selenium bioreduction can be an important control to reduce dissolved selenate concentrations. This extensive study investigated the application of Se stable isotope ratios (δ⁸²Se) of dissolved selenate to identify Se bioreduction in saturated and unsaturated mine rock piles (MRPs) located in the Elk Valley, Canada. The study included in situ and laboratory column experiments, where methanol was added to promote bioreduction. Results showed that selenate concentrations often are not reliable indicators of bioreduction. However, elevation of δ⁸²Se relative to the selenate sources in all environments provided a robust indicator of widespread selenate bioreduction. Native microbes were shown to use both methanol and natural carbon sources as an electron donor for selenate bioreduction. Variability in the magnitude of apparent isotopic fractionation in column experiments was attributed to physical and microbiological heterogeneity, while variability in apparent isotopic fractionation from in situ experiments was attributed to the entrainment of small masses of background selenate into the experiment. Due to the uncertainty in apparent epsilon values, the extent of bioreduction can only be estimated with limited confidence. Despite uncertainties, however, the application of δ⁸²Se is valuable in guiding the management of Se in mine environments.