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Updated: Jun 6, 2025

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
Published on: July 11, 2012
Reduction-responsive immobilised and protected enzymes
Congyu Wu1, Seyed Amirabbas Nazemi1, Natascha Santacroce1
1School of Life Science, University of Applied Sciences and Arts Northwestern Switzerland Hofackerstrasse 30, Muttenz CH-4132 Switzerland patrick.shahgaldian@fhnw.ch laura.suterdick@fhnw.ch.
Abstract:
We report a synthetic strategy to produce nano-immobilised and organosilica-shielded enzymes of which the biocatalytic activity is, by design, chemically enhanced under reductive conditions. The enzymes were immobilised onto silica nanoparticles through a reduction-responsive crosslinker and further shielded in an organosilica layer of controlled thickness. Under reducing conditions, disulphide bonds linking the protein to the carrier material were reduced, triggering enzyme activation. The organosilica shield prevents the enzymes from leaching from the nanobiocatalysts and preserves their integrity.
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