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Author Spotlight: Enhanced Method for Evaluating Analgesic Effects — Dual Hind Paw Carrageenan Injection in Mice
Published on: November 15, 2024
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A Modified Inflammatory Pain Model to Study the Analgesic Effect in Mice
Li Huiwen1, Zheng Baiyang1, Wei Yongkang1
1School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation, Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Yantai University.
Journal of Visualized Experiments : Jove
|December 2, 2024
Summary
Injecting carrageenan into both hind paws improves inflammatory pain models for the hot plate test. This enhanced method accurately measures analgesic effects, unlike single-paw injections.
Area of Science:
- Pharmacology
- Neuroscience
- Pain Research
Background:
- The hot plate test is a standard method for assessing pain relief.
- Carrageenan-induced inflammatory pain models are commonly used but have limitations.
- Single-hind-paw injections may not accurately reflect thermal pain thresholds due to compensatory behaviors.
Purpose of the Study:
- To develop a more accurate method for inducing inflammatory pain in mice for analgesic studies.
- To compare the efficacy of single-hind-paw versus both-hind-paw carrageenan injections.
- To validate the improved model's sensitivity to analgesic drug effects.
Main Methods:
- Mice received intraplantar injections of carrageenan in either one or both hind paws.
- Inflammatory pain was assessed using the hot plate test to measure thermal pain threshold.
- Mice were treated with celecoxib (30 mg/kg) via intragastric gavage to evaluate analgesic effects.
Main Results:
- Both-hind-paw carrageenan injection resulted in a more sensitive and reliable inflammatory pain model.
- Celecoxib significantly increased the thermal pain threshold in mice with both-hind-paw inflammation.
- Celecoxib did not show a significant effect on the thermal pain threshold in mice with single-hind-paw inflammation.
Conclusions:
- A modified both-hind-paw carrageenan injection method provides a superior model for inflammatory pain research.
- This improved model enhances the accurate evaluation of analgesic efficacy using the hot plate test.
- The findings support the use of this refined method for preclinical pain assessment.
