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Circulating microRNAs as markers for scrub typhus-associated acute kidney injury

Haeun Lee1, Jung Min Kim2, A Young Cho1

  • 1Division of Nephrology, Department of Internal Medicine, Presbyterian Medical Center, Jeonju, Republic of Korea.

Kidney Research and Clinical Practice
|December 2, 2024
PubMed
Abstract

Insights

Researchers identified specific microRNAs (miRNAs) that can detect acute kidney injury (AKI) in scrub typhus patients. These circulating miRNAs show potential as reliable biomarkers for early AKI diagnosis.

Area of Science:

  • Biomarkers
  • Molecular Biology
  • Infectious Diseases

Background:

  • Circulating microRNAs (miRNAs) are emerging biomarkers for kidney diseases.
  • Acute kidney injury (AKI) is a significant complication of scrub typhus.
  • Identifying specific biomarkers for AKI in scrub typhus is crucial for timely intervention.

Purpose of the Study:

  • To identify a circulating miRNA signature for the early detection of AKI in scrub typhus patients.
  • To differentiate miRNA profiles between scrub typhus patients with and without AKI, and healthy volunteers.
  • To explore the potential of identified miRNAs as diagnostic markers.

Main Methods:

  • Prospective enrollment of 40 scrub typhus patients (20 with AKI, 20 without) and 20 healthy volunteers.
  • Serum miRNA profiling using microarray analysis.
  • Comparative analysis of miRNA expression profiles across the three groups.

Main Results:

  • Significant differences in miRNA, small nucleolar RNA, and small Cajal body-specific ribonucleoprotein proportions were observed between patients and healthy volunteers.
  • A distinct set of 14 miRNAs showed significant upregulation or downregulation in AKI patients compared to both healthy volunteers and non-AKI patients.
  • Pathway analysis suggested the involvement of these 14 miRNAs in tumor necrosis factor alpha-related pathways.

Conclusions:

  • A specific circulating miRNA signature associated with AKI in scrub typhus patients was identified.
  • These miRNAs demonstrate predictive potential and can serve as surrogate biomarkers for scrub typhus-associated AKI.
  • The findings support the use of circulating miRNAs for non-invasive diagnosis of AKI in scrub typhus.
Keywords:
Acute kidney injuryMicroRNAsOrientia tsutsugamushiScrub typhusTumor necrosis factor-alpha

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