Oxidative cleavage of cellulose by fungi in the termite gut

Lytic polysaccharide monooxygenases (LPMOs) of auxiliary activity family 9 (AA9) oxidatively degrade cellulose. Cellulose is degraded by cellulases via hydrolysis in the termite gut. However, it remains uncertain whether oxidative cleavage of cellulose occurs within the termite gut. In this study, we report for the first time experimental support for the oxidative cleavage of cellulose in the termite (Cryptotermes declivis) gut. We identified the varieties of fungi in the termite gut through extensive analysis of the isolated fungi and sequencing of the internal transcribed spacer region. Most of the fungi were Ascomycetes. Genome sequencing revealed the presence of an AA9 LPMO (TfAA9A) in one of the isolated species, Talaromyces funiculosus. The expression of TfAA9A in the termite gut was detected using reverse transcription-polymerase chain reaction, and its ability to oxidize cellulose was confirmed in vitro through heterologous gene expression in Pichia pastoris and cellulose degradation experiments with the purified enzyme. Further transcriptome and proteomics analyses showed mRNA and protein expression of fungal AA9 LPMOs in the termite gut. These experimental data support the oxidative cleavage of cellulose in the termite gut. This study offers a new direction for understanding the mechanism of cellulose degradation in termites.