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Exploring the mechanism of Jianpi Lishi Jiedu Granules against postoperative recurrence of colorectal adenoma based on IL-6/JAK/STAT3 signaling pathway

  • 0Department of Gastroenterology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China.
Cellular Signalling +

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December 2, 2024

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December 2, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Jianpi Lishi Jiedu Granules (JLJG) prevent colorectal adenoma recurrence by inhibiting the IL-6/JAK/STAT3 inflammatory pathway. This traditional herbal formula modulates key proteins and downstream genes involved in this signaling cascade.

Area Of Science

  • Oncology
  • Immunology
  • Pharmacology

Background

  • Colorectal cancer (CRC) is a leading cause of cancer mortality globally.
  • Colorectal adenoma (CRA) recurrence post-surgery is a significant clinical concern.
  • Jianpi Lishi Jiedu Granules (JLJG) previously showed efficacy in preventing postoperative CRA recurrence.

Purpose Of The Study

  • To investigate the mechanism of JLJG in preventing postoperative CRA recurrence.
  • To determine if the JAK/STAT inflammatory signaling pathway is involved in JLJG's effect.
  • To assess the specific impact of JLJG on the JAK/STAT pathway.

Main Methods

  • Proteomics analysis to identify differentially expressed proteins (DEPs) regulated by JLJG.
  • Analysis of key proteins within the JAK/STAT signaling pathway, including IL-6, JAK1, JAK2, STAT3, CCND1, MYC, Bcl-XL, and SOCS3.
  • Assessment of downstream gene expression (BCL2, MCL1, P21) and pathway modulation.

Main Results

  • JLJG regulated 143 DEPs linked to the JAK/STAT pathway.
  • Sustained activation of the IL-6/JAK/STAT3 pathway correlated with CRA recurrence.
  • JLJG modulated key protein and downstream gene expression, inhibiting the IL-6/JAK/STAT3 pathway.

Conclusions

  • JLJG prevents postoperative CRA recurrence by inhibiting the IL-6/JAK/STAT3 signaling pathway.
  • The mechanism involves modulating key signaling proteins and downstream targets.
  • JLJG impacts both the core pathway and its negative feedback loops.

Keywords:

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