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Quantitative Proteomic Analysis of APP/PS1 Transgenic Mice
Jiayuan Wang1, Xinyu Wang1, Zihui An1
1Zhejiang Key Laboratory of Organ Development and Regeneration, School of Life and Environmental Science, Hangzhou Normal University, Hangzhou311121, China.
Current Alzheimer Research
|December 3, 2024
Summary
This study analyzed hippocampal proteomic changes in an Alzheimer
Area of Science:
- Neuroscience and Proteomics
- Investigating molecular mechanisms of neurodegenerative diseases
Background:
- Alzheimer's disease (AD) is a CNS neurodegenerative disorder with uncertain etiology.
- Key factors include amyloid-β (Aβ) plaques, tau tangles, and cholinergic deficits.
Purpose of the Study:
- To characterize hippocampal proteomic alterations in an Alzheimer's disease (AD) mouse model.
- To identify physiological and pathological shifts for potential early diagnosis and therapies.
Main Methods:
- Label-free quantitative (LFQ) proteomic analysis of the hippocampus in APP/PS1 transgenic mice.
- Comparative study with wild-type C57BL/6 mice.
- Utilized differential protein enrichment, cluster, and protein-protein interaction (PPI) network analyses.
Main Results:
- Identified 4817 proteins, with 2762 quantifiable.
- 396 proteins showed differential expression between APP/PS1 and control groups.
- 35 proteins displayed consistent temporal regulation with pathway and PPI network alterations.
Conclusions:
- Presents a comparative proteomic profile of APP/PS1 transgenic and wild-type mice.
- Details proteomic changes in the AD mouse model from 2 to 12 months.
- Offers insights into the implications of disease-associated genetic mutations.
