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Updated: Jun 6, 2025
Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
Published on: April 8, 2016
Numerical, phenotypic and functional differences in human dermal resident memory T cells between reticular dermal
Takuya Sato1, Riko Asakawa1, Youichi Ogawa1
1Department of Dermatology Faculty of Medicine University of Yamanashi Yamanashi Japan.
Background:
Resident memory T (TRM) cells and immunosuppressive Foxp3-expressing regulatory TRM (regTRM) cells are present in healthy, non-inflamed and inflamed human skin. Both types of cells are found in both the epidermis and dermis with the dermis being much thicker than the epidermis. However, it is unclear if TRM and regTRM cells differ between reticular dermal layers in terms of number, function or characteristics.
Methods:
This study examined numerical, phenotypic and functional differences in TRM and regTRM cells between the upper and lower reticular dermis in healthy, non-inflamed human skin using flow cytometry.
Results:
The phenotype and cytokine expressions of both types of cells were similar between reticular dermal layers. However, the cell count of both TRM and regTRM cells was significantly higher in the upper reticular dermis than in the lower reticular dermis.
Conclusions:
TRM and regTRM cells are phenotypically and functionally identical between reticular dermal layers. However, both types of cells are more abundant in the upper reticular dermis.
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