Plasma-activated media selectively induces apoptotic death via an orchestrated oxidative stress pathway in high-grade serous ovarian cancer cells
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View abstract on PubMed
Summary
This summary is machine-generated.Cold atmospheric pressure plasma-activated media (PAM) shows promise for treating high-grade serous ovarian cancer (HGSOC). PAM selectively targets cancer cells, inducing apoptosis and offering a potentially less toxic alternative to chemotherapy.
Area Of Science
- Oncology
- Biophysics
- Biomedical Engineering
Background
- High-grade serous ovarian cancer (HGSOC) is aggressive, often diagnosed late, and exhibits high treatment resistance.
- Current treatments like surgery and chemotherapy have severe side effects and limited efficacy in advanced stages.
- Novel, less toxic therapeutic strategies are urgently needed for HGSOC.
Purpose Of The Study
- To evaluate cold atmospheric pressure (CAP) gas discharge technology, specifically plasma-activated media (PAM), as a novel therapeutic strategy for HGSOC.
- To assess PAM's efficacy and mechanism of action in pre-clinical HGSOC models.
Main Methods
- PAM was generated using cell growth media.
- HGSOC cell lines, patient ascites cells, and primary tissue explants were treated with PAM.
- Cell viability, cell death (apoptosis), and oxidative stress were analyzed.
Main Results
- PAM demonstrated superior efficacy compared to carboplatin chemotherapy in selectively targeting ovarian cancer cells in patient samples.
- PAM induced apoptosis in HGSOC cell lines through oxidative stress and mitochondrial pathways.
- PAM was validated to selectively target tumor tissue and ascites cells.
Conclusions
- PAM is a promising therapeutic strategy for HGSOC, showing selective tumor targeting and induction of cancer cell death.
- Further investigation in in vivo models is warranted to develop intraperitoneal PAM-based therapy for HGSOC patients.
- This research supports the translation of PAM into clinical practice for ovarian cancer treatment.
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