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Pseudogene KRT17P3 Promotes NSCLC Progression Through Mir-338-3p/USP7/C-Myc

  • 0China State Institute of Pharmaceutical Industry, Shanghai, P.R. China.
Anticancer Research +

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December 3, 2024

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December 3, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

The pseudogene KRT17P3 promotes non-small cell lung cancer (NSCLC) progression by stabilizing c-Myc via the miR-338-3p/USP7 pathway. This finding highlights KRT17P3 as a potential biomarker and therapeutic target for NSCLC.

Area Of Science

  • Oncology
  • Molecular Biology
  • Cancer Genetics

Background

  • Non-small cell lung cancer (NSCLC) is a major cause of cancer mortality.
  • The molecular drivers of NSCLC remain incompletely understood.
  • The pseudogene KRT17P3 is implicated in NSCLC chemoresistance but its role in tumorigenesis is unclear.

Purpose Of The Study

  • To investigate the functional role of KRT17P3 in non-small cell lung cancer (NSCLC) development.
  • To elucidate the molecular mechanisms by which KRT17P3 influences NSCLC progression.
  • To evaluate KRT17P3 as a potential prognostic biomarker and therapeutic target for NSCLC.

Main Methods

  • In vitro assays assessed KRT17P3's impact on NSCLC cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT).
  • In vivo experiments evaluated KRT17P3's role in tumor metastasis.
  • Mechanistic studies included bioinformatics, RNA immunoprecipitation (RIP), luciferase, and chromatin immunoprecipitation (ChIP) assays.

Main Results

  • KRT17P3 significantly promotes NSCLC cell proliferation, migration, invasion, and EMT, and enhances tumor metastasis.
  • KRT17P3 stabilizes the oncogenic protein c-Myc by competitively binding miR-338-3p, upregulating deubiquitinase USP7.
  • KRT17P3 expression is upregulated by FOXA1-mediated promoter activation, driving NSCLC tumorigenesis.

Conclusions

  • KRT17P3 is a critical driver of NSCLC progression.
  • KRT17P3 regulates the USP7/c-Myc axis through miR-338-3p, impacting tumorigenesis.
  • KRT17P3 holds potential as a prognostic biomarker and therapeutic target for NSCLC.

Keywords:

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