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Updated: Jun 5, 2025
Quantitative and Temporal Control of Oxygen Microenvironment at the Single Islet Level
Published on: November 17, 2013
A microcontroller-based system for flexible oxygen control in laboratory experiments
1Behavioral Physiology, Institute of Biology, Humboldt-Universität zu Berlin, 10099 Berlin, Germany.
Abstract:
Environmental control systems are important tools for experimental researchers studying animal-environment interactions. Commercial systems for the measurement and regulation of environmental oxygen conditions are relatively expensive and cannot always be adapted to varying experimental applications. Here, I present a low-cost and highly flexible oxygen control system using Arduino microcontrollers in combination with a commercial optical oxygen sensor. Hardware and software examples are provided for three different applications: single-setpoint, sequential and long-term dissolved oxygen (DO) control. All tested control systems created the desired DO conditions with high accuracy and repeatability across trials. The resources provided shown here can be adapted and modified to be used in a variety of experimental contexts.
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