The renaissance of multipurpose AMeX method over conventional tissue processing method in the current diagnostic era:...

Area of Science:

• Histopathology
• Molecular Biology
• Biochemistry

Background:

• Traditional tissue fixation and processing methods for H&E staining, IHC, and DNA extraction are often complex and time-consuming.
• The acetone-methyl benzoate-xylene (AMeX) method is emerging as a simpler alternative to aqueous formaldehyde fixation and alcohol dehydration.

Purpose of the Study:

• To evaluate the efficacy of the AMeX method for tissue processing.
• To compare the quality of H&E staining, IHC, and genomic DNA extracted from AMeX-processed human oral mucosal lesions with conventional methods.

Main Methods:

• Human oral mucosal lesions (≤1 cm) were processed using the AMeX method.
• Tissues were stained with H&E and subjected to IHC.
• Genomic DNA was extracted from AMeX-processed tissues.
• Results were compared to conventionally processed tissues.

Main Results:

• H&E staining quality was comparable between AMeX and conventional methods.
• AMeX processing resulted in improved IHC staining quality.
• Both the quality and quantity of genomic DNA extracted were enhanced with the AMeX method.

Conclusions:

• The AMeX method is an effective and simplified approach for tissue processing.
• AMeX offers advantages for subsequent H&E staining, IHC, and genomic DNA extraction, particularly in terms of improved IHC quality and DNA yield.