Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Biofilms01:29

Biofilms

Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Friends and neighbours as non-kin caregivers of seriously ill patients at the end of life: a scoping review of experiences, individual capacities, support needs and services.

BMC palliative care·2025
Same author

The Submucosal Microbiome Correlates with Peri-implantitis Severity.

Journal of dental research·2025
Same author

In-vitro study of the wear behavior of telescopic crowns made of CoCr, zirconia or PEEK.

Journal of materials science. Materials in medicine·2025
Same author

Future Directions of the Prokaryotic Chromosome Field.

Molecular microbiology·2025
Same author

Shear bond strength between dental adhesive systems and an experimental niobium-based implant material.

Journal of materials science. Materials in medicine·2024
Same author

Impact of loading protocol of a mandibular single implant-supported complete denture on oral health-related quality of life over a period of 5 years: A randomized controlled trial.

Journal of dentistry·2023

Related Experiment Video

Updated: Jun 5, 2025

Oral Biofilm Formation on Different Materials for Dental Implants
11:19

Oral Biofilm Formation on Different Materials for Dental Implants

Published on: June 24, 2018

11.4K

Gradual Acidification at the Oral Biofilm-Implant Material Interface.

K Doll-Nikutta1,2, S C Weber1,2, C Mikolai1,2

  • 1Department for Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover, Germany.

Journal of Dental Research
|December 4, 2024
PubMed
Summary

Dental implant biofilms lower pH at the material interface, especially with sufficient nutrients. This pH drop can trigger antibacterial release systems to prevent implant loss.

Keywords:
confocal microscopydental implantdental plaquehydrogen-ion concentrationnutrientstitanium

More Related Videos

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries
08:20

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries

Published on: March 31, 2021

6.3K
Ratiometric Imaging of Extracellular pH in Dental Biofilms
13:05

Ratiometric Imaging of Extracellular pH in Dental Biofilms

Published on: March 9, 2016

10.3K

Related Experiment Videos

Last Updated: Jun 5, 2025

Oral Biofilm Formation on Different Materials for Dental Implants
11:19

Oral Biofilm Formation on Different Materials for Dental Implants

Published on: June 24, 2018

11.4K
Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries
08:20

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries

Published on: March 31, 2021

6.3K
Ratiometric Imaging of Extracellular pH in Dental Biofilms
13:05

Ratiometric Imaging of Extracellular pH in Dental Biofilms

Published on: March 9, 2016

10.3K

Area of Science:

  • Biomaterials science
  • Microbiology
  • Dental research

Background:

  • Oral biofilm colonization on dental implants can cause inflammation and implant failure.
  • Developing smart implant surfaces that release antimicrobials upon bacterial detection is crucial.
  • Local pH changes due to bacterial metabolism are a promising trigger for such systems.

Purpose of the Study:

  • To precisely measure the pH at the interface between dental implant materials and oral biofilms.
  • To understand pH dynamics in different biofilm types (monospecies, multispecies, in vitro, in situ).
  • To identify pH thresholds for triggering antibacterial release from smart implant surfaces.

Main Methods:

  • Utilized in vitro (Streptococcus oralis) and in situ biofilms on titanium discs and splints.
  • Characterized biofilm morphology using live/dead fluorescence staining.
  • Quantified extracellular pH gradients within biofilms using pH-sensitive dye (C-SNARF-4) and confocal laser-scanning microscopy.

Main Results:

  • All mature biofilms exhibited a pH gradient, with the lowest pH at the material interface.
  • Biofilm pH decreased over time and with nutrient availability, independent of biofilm source or donor.
  • Initial mild acidification (approx. pH 6.3) intensified to approx. pH 5.0 with prolonged nutrient exposure.

Conclusions:

  • The material-biofilm interface experiences significant, nutrient-dependent acidification.
  • pH 5.0 represents a key threshold for triggering antibacterial release systems.
  • This pH-dependent acidification allows for tailored antimicrobial release based on biofilm maturity.