Spatial Transcriptome and Single Nucleus Transcriptome Sequencing Reveals Tetrahydroxy Stilbene Glucoside Promotes...

Area of Science:

• Reproductive biology
• Stem cell research
• Pharmacology

Background:

• Ovarian dysfunction is a primary cause of female infertility.
• Improving ovarian function is crucial for preventing and treating infertility.
• Plant-derived compounds offer potential therapeutic avenues.

Purpose of the Study:

• To screen plant-derived compounds for in vitro activity on ovarian organoids.
• To elucidate the mechanism by which Tetrahydroxy stilbene glucoside (TSG) promotes ovarian organoid development.
• To establish a platform for screening infertility treatments.

Main Methods:

• In vitro screening of plant compounds on ovarian organoids derived from feeder-free female germline stem cells.
• Single nucleus transcriptome sequencing and spatial transcriptome sequencing.
• Analysis of cell types, subtypes, transcription factors, pseudo-time, and cell communication dynamics.

Main Results:

• Tetrahydroxy stilbene glucoside (TSG) was identified as a promoter of ovarian organoid development.
• TSG facilitates ovarian organoid development via the VEGF-A/EphB2 signaling pathway between granulosa cells and oocytes.
• A comprehensive spatiotemporal map of TSG's effects was generated.

Conclusions:

• TSG promotes ovarian organoid development through specific cell-cell communication pathways.
• This study provides a platform for discovering novel infertility treatments.
• Findings support the potential clinical application of plant-derived compounds for ovarian dysfunction.