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Smartphone-Enabled Point-of-Care Testing for Prehospital Stroke Diagnosis
Paloma Menéndez-Valladares1,2,3, Rosa M Delgado1, David Núñez-Jurado1,3
1Neurovascular Research Group, Institute de Biomedicine of Seville, IBiS/Virgen Macarena University Hospital/CSIC/University of Seville, Seville, Spain.
Objectives:
The objective of this study was to evaluate the feasibility of point-of-care testing (POCT) devices for N-terminal pro-B-type natriuretic peptide (NT-proBNP) measurement in prehospital settings, with the aim of improving the speed and accuracy of stroke diagnosis, thereby facilitating quicker and more effective patient care.
Methods:
Prehospital blood samples were collected from suspected stroke patients, and NT-proBNP levels were measured using a POCT device in ambulances and hospitals. Results from the NT-proBNP POCT and smartphone images were analyzed. Plasma samples underwent Elecsys proBNP II immunoassay after storage at -80ºC.
Results:
A total of 121 suspected stroke patients were included in the study. The correlation between POCT measured by the POCT and immunoassay for NT-proBNP was strong (R = 0.926). Smartphone images also strongly correlated with POCT values at 10 min (R²=0.9716) and 15 min (R²=0.9405). Stability analysis of samples showed consistent NT-proBNP results and a high correlation (R = 0.907) was observed between plasma and whole blood samples for NT-proBNP POCT.
Conclusions:
This study highlights the potential of NT-proBNP POCT devices in ambulances to expedite stroke diagnosis and management within 10 min. Smartphone integration further enhances efficiency, adding advancement in prehospital stroke management.
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