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Updated: Jun 5, 2025

Characterization of the Isolated, Ventilated, and Instrumented Mouse Lung Perfused with Pulsatile Flow
Published on: April 29, 2011
Effect of fluid and driving pressure on cyclical "on-off" flow of pulmonary microcirculation during mechanical
Siyi Yuan1, Xiangyu Chen1, Liangyu Mi1
1Department of Critical Care Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Dongcheng District, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Science, 1 Shuaifuyuan, Beijing, China.
Objectives:
This study aimed to identify the cyclical "on-off" flow of pulmonary microcirculation during inspiration and expiration by sidestream dark field imaging (SDF) technology in vivo and investigate the effects of volume status and driving pressure on cyclical "on-off" flow of microcirculation.
Methods:
24 ARDS-modeled rabbits were randomly divided into high-driving pressure group (HDP group) and low-driving pressure group (LDP group). Lung microcirculation measurements were performed using the SDF microscope at two timepoints (T1 CVP 2-4 mmHg, T2 CVP 8-10 mmHg). From T1 to T2, 10 ml/kg saline was infused to increase CVP. The cyclical "on-off" pulmonary microcirculation was quantitatively assessed by the change of microcirculation between expiration and inspiration.
Results:
Proportion of perfused vessels (PPV), microvascular flow index (MFI), perfused vessel density (PVD), and total vessel density (TVD) at expiration were significantly higher than inspiration in the HDP group. The HDP group has a higher ΔPPV and ΔPVD. After fluid loading, ΔPPV and ΔMFI decreased. TNF-α, IL-6, Ang-2, and vWF levels in the HDP group were higher. The HDP group also has a higher lung wet-weight/body weight ratio, lung wet-to-dry weight ratio, and more severe damage of pulmonary capillaries than the LDP group.
Conclusions:
The difference in alveolar perfused microcirculation between inspiration and expiration defined as cyclical "on-off flow" can be detected. High driving pressure can enhance the cyclical "on-off" flow, and fluid loading can relieve it. High driving pressure can potentially cause injury to pulmonary capillaries due to the phenomenon of "on-off" flow, thereby exacerbating ARDS.
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