Colorimetric quantitative detection of steroid hormones using an indicator displacement assay-based chemosensor array

Area of Science:

• Analytical Chemistry
• Biomedical Sensing
• Hormone Analysis

Background:

• Accurate detection of sex hormones is crucial for diagnosing various endocrine disorders.
• Existing methods for hormone detection can be complex and require specialized equipment.
• A need exists for simpler, more accessible methods for hormone analysis.

Purpose of the Study:

• To develop a colorimetric chemosensor array for visualizing and quantifying sex hormones.
• To demonstrate the array's capability in detecting steroid hormones in complex biological matrices like saliva.

Main Methods:

• Utilized an indicator displacement assay within a chemosensor array format.
• Employed colorimetric detection for visual and quantitative analysis of hormone mixtures.
• Tested the sensor's efficacy in detecting a specific steroid hormone in diluted human saliva.

Main Results:

• The chemosensor array successfully visualized structural differences between various hormones.
• Quantitative detection of sex hormones within mixtures was achieved.
• The array demonstrated the ability to detect a steroid hormone in diluted human saliva.

Conclusions:

• The developed colorimetric chemosensor array offers a promising tool for hormone analysis.
• This technology enables both qualitative and quantitative detection of sex hormones.
• The sensor's application in analyzing saliva highlights its potential for non-invasive diagnostic applications.