Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

8.1K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.1K
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

244
Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
244
Photoluminescence: Applications01:14

Photoluminescence: Applications

374
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
374
Flame Photometry: Lab01:16

Flame Photometry: Lab

213
In a flame photometer, when a solution like potassium chloride is aspirated into the flame, the solvent evaporates, leaving behind dehydrated salt. This salt dissociates into free gaseous atoms in their ground state. Some of these atoms absorb energy from the flame, leading to their excitation. The excited atoms return to the ground state, emitting photons at characteristic wavelengths. Because only electronic transitions are involved, the resulting emission lines are very narrow. The intensity...
213
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

540
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
540

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In Situ Physicochemical Characterization of Secondary Organic Aerosols via Fluorescence Probe Spectroscopy.

Environmental science & technology·2026
Same author

Ratiometric Optical Sensing of Aerosol Phase State with Excited-State Intramolecular Proton Transfer Probes.

Analytical chemistry·2025
Same author

Second harmonic generation null angle polarization analysis for determining interfacial potential at charged interfaces.

The Journal of chemical physics·2024
Same author

Relative Humidity-Dependent Phase Transitions in Submicron Respiratory Aerosols.

The journal of physical chemistry. A·2024
Same author

Prediction of the Response of a Photoionization Detector to a Complex Gaseous Mixture of Volatile Organic Compounds Produced by α-Pinene Oxidation.

ACS earth & space chemistry·2023
Same author

Size Dependence of Liquid-Liquid Phase Separation by in Situ Study of Flowing Submicron Aerosol Particles.

The journal of physical chemistry. A·2023

Related Experiment Video

Updated: Jun 5, 2025

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments
07:51

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Published on: December 21, 2017

8.2K

Aerosol Fluorescent Labeling via Probe Molecule Volatilization.

Angel M Gibbons1, Michael Boadu1, Paul E Ohno1

  • 1Department of Chemistry and Biochemistry, Auburn University, Auburn, Alabama 36849, United States.

Analytical Chemistry
|December 4, 2024
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method to study aerosol properties using fluorescence. This technique allows labeling of particles without pre-mixing, enabling analysis of respiratory and atmospheric aerosols.

More Related Videos

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

22.1K
Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization
10:01

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

Published on: July 30, 2020

7.1K

Related Experiment Videos

Last Updated: Jun 5, 2025

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments
07:51

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Published on: December 21, 2017

8.2K
Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

22.1K
Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization
10:01

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

Published on: July 30, 2020

7.1K

Area of Science:

  • Aerosol science
  • Analytical chemistry
  • Spectroscopy

Background:

  • Physicochemical properties of aerosols are crucial for various applications.
  • Measuring these properties is challenging due to particle size and density.
  • Fluorescence probe spectroscopy is a powerful in situ technique, but limited by pre-mixing requirements.

Purpose of the Study:

  • To develop a novel method for fluorescent labeling of aerosols via probe molecule volatilization.
  • To overcome limitations of pre-mixing in fluorescence probe spectroscopy for aerosol analysis.
  • To enable in situ characterization of respiratory and atmospheric aerosols.

Main Methods:

  • Developed a volatilization-based method for aerosol fluorescent labeling.
  • Utilized polarity-sensitive probes (Nile red and Prodan) for labeling polyethylene glycol (PEG) aerosols.
  • Validated the method by comparing fluorescence emission of prelabeled and volatilized-probe PEG particles.

Main Results:

  • Successfully labeled model PEG aerosols using probe molecule volatilization.
  • Demonstrated the method's validity through consistent relative humidity-dependent fluorescence.
  • Preliminary application showed indication of hygroscopicity in artificial saliva and secondary organic aerosols.

Conclusions:

  • The developed volatilization method enables fluorescent labeling of aerosols without pre-mixing.
  • This technique expands the applicability of fluorescence probe spectroscopy to challenging aerosol types.
  • Paves the way for studying exhaled and natural aerosols where prelabeling is not feasible.