Related Experiment Video
Updated: Jun 5, 2025

Measurements of Physiological Stress Responses in C. Elegans
Published on: May 21, 2020
Functional Characterization of RseC in the SoxR Reducing System and Its Role in Oxidative Stress Response in
Kang-Lok Lee1, Joon-Hee Lee2, Yun-Hee Kim1
1Department of Biology Education, IALS, Gyeongsang National University, Gyeongsang National University, Jinju 52828, Republic of Korea.
Abstract:
The reducing system of SoxR consists of a putative electron transfer system encoded by the rsxABCDGE operon, RseC encoded from the unlinked rpoE-rseABC operon, and ApbE. RseC is composed of two transmembrane helices, with both the N-terminal and C-terminal domains located in the cytoplasm. The N-terminal domain has a four-cysteine motif, CX5CX2CX5C, in the cytoplasm, with the latter three cysteines highly conserved in RseC homologs, allowing the SoxR reducer complex to function in Escherichia coli. These three cysteines can form an oxygen-sensitive Fe-S cluster when only the N-terminal domain is expressed in a truncated form. Without the C-terminal domain, RseC shows no significant difference in interaction with the SoxR reducer complex, but its ability to complement the function of an rseC mutant is greatly reduced. Additionally, the rseC mutant exhibits weak resistance to cumene hydrogen peroxide in the stationary phase and increased sensitivity to hydrogen peroxide in the exponential phase, independent of the SoxR regulon. This suggests that the full-length sequence of RseC is essential for its function and that it may have SoxR-independent additional roles.
More Related Videos
Related Concept Videos
Transcriptional Regulation: Riboswitches
Protein Modifications in the RER
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
Regulation of the Unfolded Protein Response
Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Riboswitches
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Redox Reactions

