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Ascorbic acid transport in mammalian kidney.

R C Rose

    The American Journal of Physiology
    |April 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    The kidney reabsorbs ascorbic acid (vitamin C) through a sodium-dependent active transport mechanism. This process concentrates vitamin C within kidney cells, preventing its loss in urine.

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    Area of Science:

    • Nephrology
    • Biochemistry
    • Cell Biology

    Background:

    • Ascorbic acid circulates freely in plasma and is filtered by the kidney.
    • Renal reabsorption prevents urinary loss of ascorbic acid.
    • The specific mechanism for renal ascorbic acid transport was previously unknown.

    Purpose of the Study:

    • To elucidate the mechanism of renal ascorbic acid transport.
    • To investigate the active transport of ascorbic acid in kidney tissue.

    Main Methods:

    • Incubation of rat and guinea pig kidney slices and isolated tubules in vitro.
    • Use of low concentrations of radiolabeled [14C]ascorbic acid.
    • Assessment of uptake under conditions of altered sodium concentration, metabolic inhibition, and temperature changes.

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    Main Results:

    • Kidneys of both species handled ascorbic acid similarly.
    • Ascorbic acid accumulated in renal tissue to 3-4 times the bathing media concentration.
    • Uptake was reduced by sodium replacement (Li+, Cs+), metabolic inhibitors, and low temperatures, indicating active, sodium-dependent transport.
    • Ascorbic acid was predominantly nonmetabolized during absorption.

    Conclusions:

    • Ascorbic acid is reabsorbed in the kidney via a sodium-dependent active transport mechanism.
    • This transport concentrates ascorbic acid within renal cells.
    • The study confirms functional brush-border membrane transport in isolated renal preparations.