Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

386
Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
386
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

191
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
191
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

900
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
900
Atomic Absorption Spectroscopy: Interference01:25

Atomic Absorption Spectroscopy: Interference

663
Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
663

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Excellent Overall Water Splitting Catalyst of Single Phase 6H Triple Perovskite Ba<sub>3</sub>Co(Co<sub>0.25</sub>Ru<sub>0.75</sub>)<sub>2</sub>O<sub>9</sub> at 1 A cm<sup>-2</sup> for 1000 h.

Angewandte Chemie (International ed. in English)·2026
Same author

Targeting the cytokine-epigenetic axis: a new paradigm and prospects for disease treatment.

European cytokine network·2026
Same author

Protective Effects of Tuber sinoaestivum-Derived Exosome-Like Nanovesicles Against Cisplatin-Induced Acute Liver Injury and Acute Kidney Injury.

Journal of food science·2026
Same author

A palmitoylation signature for prognosis immune infiltration and drug sensitivity in esophageal carcinoma.

iScience·2026
Same author

Development of the Online Standard Addition Method for Stable Isotope Analysis and Its Validation for Sulfur Isotope Determination.

Analytical chemistry·2026
Same author

Applications of carbon-based materials in photothermal therapy for drug-resistant tumors: a review of research from synergistic therapy to theranostics.

Biomaterials science·2026

Related Experiment Video

Updated: Jun 5, 2025

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
09:24

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: December 18, 2020

5.5K

Correction Strategies for Isotopic Ratios in Overspiked Samples Using the Double Spike Technique.

Jian-Ming Zhu1, Wenlong Sun1, Thomas M Johnson2

  • 1State Key Laboratory of Geological Processes and Mineral Resources, and Frontiers Science Center for Deep-time Digital Earth, China University of Geosciences (Beijing), Beijing 100083, China.

Analytical Chemistry
|December 5, 2024
PubMed
Summary

A new mathematical method corrects overspiked isotope ratio measurements using the double spike (DS) technique. This approach avoids sample reprocessing, saving time and resources in isotope analysis.

More Related Videos

Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis
07:57

Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis

Published on: August 15, 2018

13.9K
Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
12:47

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources

Published on: January 22, 2018

9.3K

Related Experiment Videos

Last Updated: Jun 5, 2025

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
09:24

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: December 18, 2020

5.5K
Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis
07:57

Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis

Published on: August 15, 2018

13.9K
Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
12:47

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources

Published on: January 22, 2018

9.3K

Area of Science:

  • Geochemistry
  • Analytical Chemistry
  • Isotope Geochemistry

Background:

  • The double spike (DS) technique is crucial for accurate isotope ratio measurements.
  • Overspiking, where samples contain excessive spike material, necessitates reprocessing, leading to time and resource inefficiencies.

Purpose of the Study:

  • To introduce a mathematical scheme for correcting isotope ratios in overspiked samples.
  • To eliminate the need for repurification and reanalysis of overspiked samples.

Main Methods:

  • A straightforward mathematical model was developed to rectify isotope ratios of overspiked samples.
  • The method involves adding a standard solution to bring the overspiked ratio into the normal range.
  • Theoretical equations and an error propagation model were established and applied to nickel (Ni) isotopes as a case study.

Main Results:

  • The proposed method accurately determines actual sample isotopes using spike-to-sample ratios and mixture isotope ratios.
  • Accuracy and precision comparable to traditional DS measurements were achieved for Ni, chromium (Cr), and cadmium (Cd) isotopes.
  • Effective correction was demonstrated when the added standard fraction (f_std) was ≤0.60 or the overspiked multiple was ≤2.5.

Conclusions:

  • This mathematical scheme provides a robust solution for overspiked samples in DS isotope analysis.
  • The method expands the applicability of DS technique to a wider range of sample conditions and elements.
  • It offers a more efficient alternative to traditional reprocessing methods for overspiked samples.