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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
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Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

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Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
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Updated: Jun 5, 2025

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
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Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

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Towards Next Generation Protein Sequencing.

Yakun Yi1,2, Ziyi Li1,2, Lei Liu3

  • 1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, 100190, Beijing, China.

Chembiochem : a European Journal of Chemical Biology
|December 5, 2024
PubMed
Summary
This summary is machine-generated.

Advancements in protein sequencing, including nanopore sensing, offer new ways to read peptide sequences. This technology promises to revolutionize biological understanding and clinical diagnostics.

Keywords:
nanoporeprotein sequencingsingle-molecule analysis

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Protein structure and function are central to life sciences.
  • Edman degradation and mass spectrometry are established protein sequencing methods.
  • Single-molecule technologies are emerging as powerful tools in protein analysis.

Purpose of the Study:

  • To review the advancements in protein sequencing technologies.
  • To highlight the role of single-molecule technologies, particularly nanopore sensing.
  • To discuss the future potential of next-generation protein sequencing.

Main Methods:

  • Review of historical protein sequencing techniques (Edman degradation, mass spectrometry).
  • Focus on recent applications of single-molecule technologies.
  • Detailed examination of nanopore sensing for amino acid and peptide analysis.

Main Results:

  • Nanopore sensing demonstrates reliability in single-molecule analysis.
  • Significant progress in amino acid recognition and short peptide differentiation using nanopore technology.
  • Demonstrated capability for accurate peptide sequence reading with nanopore sensing.

Conclusions:

  • Nanopore sensing is a key innovation in single-molecule protein sequencing.
  • Next-generation sequencing technologies will enhance understanding of biological mechanisms.
  • These advancements are expected to significantly improve clinical diagnostics and treatments.