Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

POSTN<sup>+</sup> CAFs facilitate gastric cancer peritoneal metastasis by promoting ICAM-1-dependent tumor cell adhesion and CD8<sup>+</sup> T-cell exhaustion.

Frontiers in immunology·2026
Same author

MPLIF: Multi-parametric leaky integrate-and-fire neuron for spiking neural networks.

Neural networks : the official journal of the International Neural Network Society·2026
Same author

Photodynamic Effect of Bioactive Titanium Metal Initiated by X-Ray and Its Potential Application for Bone Tumor Treatment.

International journal of cancer·2026
Same author

Unraveling the regulatory role of intercellular communication in intestinal immune cells mediated by H₂ in sepsis recovery through single-cell RNA sequencing.

Journal of translational medicine·2026
Same author

Insights into efficient activation of H<sub>2</sub>O<sub>2</sub> by sulfur-doped CuFeO<sub>2</sub>: Important role of sulfur and sulfamethoxazole oxidation.

Journal of colloid and interface science·2026
Same author

Field-induced multimode liquid crystal switchable grating based on patterned alignment.

Optics letters·2026

Related Experiment Video

Updated: Jun 5, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

8.9K

Fluorescence engineering in metamaterial-assisted super-resolution localization microscope.

Kyu Ri Choi1, Shilong Li2, Igor Ozerov3

  • 1Department of Physics, Chungbuk National University, Cheongju, Chungbuk 28644, South Korea.

Nanophotonics (Berlin, Germany)
|December 5, 2024
PubMed
Summary
This summary is machine-generated.

Metamaterial-assisted super-resolution microscopy uses cyclic group metasurfaces to enhance fluorescence. This technique achieves 0.9-nm localization accuracy, improving signal-to-noise ratio for advanced imaging.

Keywords:
Purcell effectenhanced fluorescencemetamaterialssuper-resolution imaging

More Related Videos

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

9.8K
Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy
13:13

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

Published on: December 3, 2012

16.1K

Related Experiment Videos

Last Updated: Jun 5, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

8.9K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

9.8K
Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy
13:13

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

Published on: December 3, 2012

16.1K

Area of Science:

  • Optics and Photonics
  • Materials Science
  • Biophysics

Background:

  • Single-molecule localization microscopy (SMLM) offers sub-diffraction-limit resolution.
  • Conventional SMLM requires specific probes and conditions.
  • Metamaterial-assisted microscopy offers broader applicability with various fluorophores.

Purpose of the Study:

  • To investigate fluorescence engineering in metamaterial-assisted localization microscopy.
  • To explore the use of cyclic group metasurfaces for enhanced super-resolution imaging.
  • To demonstrate tailored control over fluorophore photophysics.

Main Methods:

  • Fabrication of cyclic group metasurfaces coated with fluorescent films.
  • Characterization of fluorophore photoluminescence intensity and photobleaching lifetime.
  • Application of spatially varied Purcell effect for fluorescence enhancement.
  • Implementation of metamaterial-assisted super-resolution localization microscopy.

Main Results:

  • Demonstrated spatially varied Purcell effect near metasurfaces.
  • Observed tailored variations in fluorophore photoluminescence intensity and photobleaching lifetime.
  • Achieved enhanced fluorophore emission and altered blinking dynamics.
  • Obtained a super-resolution image with 0.9-nm localization accuracy.
  • Increased signal-to-noise ratio due to enhanced fluorescence.

Conclusions:

  • Metamaterial-assisted microscopy enables super-resolution imaging under general conditions.
  • Cyclic group metasurfaces effectively engineer fluorescence via the Purcell effect.
  • This approach significantly improves localization accuracy and signal-to-noise ratio.
  • The findings advance light-matter interaction control beyond the diffraction limit.