Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

4.6K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
4.6K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

2
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
2

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantifying 3D live-cell membrane dynamics using dynamic metal-induced energy transfer spectroscopy (dynaMIET).

Science advances·2026
Same author

All-Optical Nonlinear Real and Fourier-Space Shaping with All-Dielectric Fano Resonant Metasurfaces.

ACS nano·2026
Same author

Mapping Optical Chirality with Single Fluorescent Molecules.

Nano letters·2026
Same author

Versatile Microfluidics Platform for Enhanced Multitarget Super-Resolution Microscopy.

ACS nano·2026
Same author

Influence of Driving Pulse Properties on Third-Harmonic Diffraction from Quasi-BIC Metasurfaces.

ACS photonics·2025
Same author

Information advantage in sensing revealed by Fano-resonant Fourier scatterometry.

Nature communications·2025

Related Experiment Video

Updated: Jun 5, 2025

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.0K

Super-resolution imaging: when biophysics meets nanophotonics.

A Femius Koenderink1, Roman Tsukanov2, Jörg Enderlein2,3

  • 1Center for Nanophotonics, AMOLF, Science Park 104, 1098 XG Amsterdam, The Netherlands.

Nanophotonics (Berlin, Germany)
|December 5, 2024
PubMed
Summary

Super-resolution microscopy techniques like single-molecule localization microscopy (SMLM) are advancing nanophotonics and biophysics. This review explores how SMLM leverages nanophotonics for new insights and how nanophotonics can improve microscopy for biological studies.

Keywords:
fluorescence-lifetime imaging microscopylocal density of stateslocalization artifactsmetal-induced energy transferquantum yieldsingle-molecule localization microscopy

More Related Videos

Super-resolution Imaging of the Bacterial Division Machinery
08:47

Super-resolution Imaging of the Bacterial Division Machinery

Published on: January 21, 2013

11.8K
Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
12:44

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

19.9K

Related Experiment Videos

Last Updated: Jun 5, 2025

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.0K
Super-resolution Imaging of the Bacterial Division Machinery
08:47

Super-resolution Imaging of the Bacterial Division Machinery

Published on: January 21, 2013

11.8K
Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
12:44

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

19.9K

Area of Science:

  • Optics and Photonics
  • Biophysics
  • Materials Science

Background:

  • Probing light-matter interactions at the nanometer scale is crucial for fields like nanophotonics, quantum electrodynamics, and biosensing.
  • Innovations in microscopy, particularly super-resolution single-molecule localization microscopy (SMLM), enable observation of phenomena across multiple length scales.
  • SMLM has revolutionized bioimaging by providing unprecedented resolution for studying intracellular molecular mechanisms.

Purpose of the Study:

  • To review recent advances in nanophotonics utilizing SMLM.
  • To demonstrate how nanophotonics concepts can enhance biophysics microscopy techniques.
  • To explore the synergy between localization microscopy and nanophotonics for future research.

Main Methods:

  • Introduction to the fundamental concepts and measurable observables of SMLM.
  • Linking SMLM observables to physical quantities relevant in biophysics and nanophotonics.
  • Description of state-of-the-art experiments applying SMLM in nanophotonics.

Main Results:

  • Discussion of localization artifacts arising from fluorescent emitter-resonant medium interactions and potential solutions.
  • Demonstration of using fluorescent emitter-plasmonic structure interactions for cell profiling and membrane organization studies.
  • Highlighting the successful application of SMLM in nanophotonics research.

Conclusions:

  • The integration of SMLM and nanophotonics offers powerful tools for fundamental research and biological applications.
  • Synergistic developments in these fields promise to unlock new research directions.
  • This review provides a comprehensive overview of current progress and future potential.