LAMP5, One of Four Genes Related to Oxidative Stress That Predict Biochemical Recurrence-Free Survival, Promotes Proliferation and Invasion in Prostate Cancer
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View abstract on PubMed
Summary
This summary is machine-generated.This study identifies LAMP5 as a key gene in prostate cancer (PCa) development linked to oxidative stress. A new recurrence score model highlights LAMP5
Area Of Science
- Oncology
- Genetics
- Biochemistry
Background
- Prostate cancer (PCa) progression is significantly influenced by elevated oxidative stress (OS) and compromised antioxidant defenses.
- Identifying genes involved in OS is crucial for advancing PCa treatment strategies and research directions.
Purpose Of The Study
- To identify genes associated with oxidative stress in prostate cancer.
- To develop a recurrence score (RS) model for predicting PCa outcomes.
- To investigate the role of LAMP5 in PCa proliferation and invasion.
Main Methods
- Utilized bulk and single-cell RNA-seq data from TCGA, GTEx, and GEO databases.
- Applied R packages like Seurat, AUCell, and limma for data analysis, including ROS marker identification and differential gene expression.
- Developed a recurrence score (RS) model using LASSO regression and explored tumor immune microenvironment and genetic mutations.
Main Results
- A robust RS model was established as an independent predictor for PCa.
- Significant differences in tumor immune microenvironment and gene mutation frequencies were observed between high- and low-risk groups.
- Downregulation of LAMP5 inhibited cell proliferation and invasion in PCa cell lines (PC-3 and DU145) in vitro.
Conclusions
- The developed RS model effectively predicts PCa recurrence.
- LAMP5 plays a significant role in promoting prostate cancer cell proliferation and invasion.
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