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Related Concept Videos

Pathophysiology of Peptic Ulcer Disease: Mucosal Defense Factors01:24

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Peptic ulcer disease, commonly called PUD, represents a multifaceted condition characterized by disruptions in the lining of the gastrointestinal (GI)  tract. Central to the protection of the gastrointestinal lining is the mucosal-bicarbonate barrier. This physiological defense mechanism is a formidable shield against the corrosive effects of gastric acid and pepsin secretion in the stomach. Its role is pivotal in maintaining the structural integrity of the stomach's inner lining.
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The gastric glands contain parietal cells that secrete hydrochloric acid (HCl) for digestion. The cells secrete HCl because it is highly corrosive and essential for breaking down food. To achieve this, they secrete hydrogen and chloride ions into the lumen of the gastric glands, which combine to form HCl.
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Related Experiment Video

Updated: Jun 5, 2025

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Mucosal Hub Bacteria as Potential Targets for Improving High-Fat Diet-Related Intestinal Barrier Injury.

Li Shao1,2,3, Binbin Zhang1,3, Yu Song4

  • 1School of Clinical Medicine, Hangzhou Normal University, The Affiliated Hospital of Hangzhou Normal University, Hangzhou 311121, Zhejiang, China.

The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Medicale
|December 5, 2024
PubMed
Summary

High-fat diets damage the intestinal barrier, increasing disease risk. Targeting specific gut bacteria, like Bacteroides ovatus, may offer a novel therapeutic strategy to restore intestinal barrier function.

Keywords:
high-fat diethub bacteriaintestinal barrier injurymucosal microbiome

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Area of Science:

  • Microbiology
  • Gastroenterology
  • Metabolomics

Background:

  • Intestinal barrier dysfunction is linked to obesity and diabetes, with limited treatment options.
  • The gut microbiome and intestinal barrier exhibit close interactions, suggesting microbial targets for diet-induced injury.

Purpose of the Study:

  • To investigate mucosal bacteria as potential targets for high-fat diet (HFD)-induced intestinal barrier injury.
  • To characterize changes in the gut microbiome composition and network structure in response to HFD.

Main Methods:

  • Whole-genome metagenomics to analyze mucosal microbiome composition.
  • Assessment of intestinal barrier integrity using serum lipopolysaccharide (LPS), FITC-dextran intensity, and ZO-1 protein expression.
  • Correlation analysis between microbial changes and physiological/biochemical markers.

Main Results:

  • HFD induced obesity, altered lipid profiles, and confirmed intestinal barrier injury (elevated LPS, FITC-dextran, reduced ZO-1).
  • Significant shifts in mucosal microbiome composition, including a decrease in hub bacteria like Bacteroides ovatus, were observed in HFD-fed mice.
  • Decreased Bacteroides ovatus abundance correlated with increased intestinal permeability and altered metabolic pathways, such as "Mitochondrial biogenesis".

Conclusions:

  • Mucosal hub bacteria, particularly Bacteroides ovatus, represent promising therapeutic targets for ameliorating HFD-related intestinal barrier dysfunction.
  • Microbiome alterations provide insights into the pathogenesis of HFD-induced metabolic and intestinal diseases.