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Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy
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Time-Multiplexed Miniaturized Two-Photon Microscopy
Shing-Jiuan Liu1, Zixiao Zhang1, Ben Mattison2,3
1Department of Electrical and Computer Engineering, University of California, Davis, Davis, CA 95616, USA.
Abstract:
We propose a time-multiplexed miniaturized two-photon microscope (TM-MINI2P), enabling a two-fold increase in imaging speed while maintaining a high spatial resolution. Using TM-MINI2P, we conducted high-speed in-vivo calcium imaging in mouse cortex.

