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Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Jun 5, 2025

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

Super-resolution optical microscopy using cylindrical vector beams.

Min Liu^1,2, Yunze Lei¹, Lan Yu¹

¹School of Physics, Xidian University, Xi'an 710071, China.

Nanophotonics (Berlin, Germany)

|December 5, 2024

Summary

This summary is machine-generated.

Cylindrical vector beams (CVB) enhance super-resolution microscopy, enabling finer details in nanomaterials and nanobiology. This review covers CVB strategies and recent advances in super-resolution imaging techniques.

Keywords:

cylindrical vector beam far-field microscopy near-field microscopy super-resolution microscopy

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Last Updated: Jun 5, 2025

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

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Published on: November 16, 2019

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

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Published on: September 8, 2017

Area of Science:

Optical microscopy
Nanotechnology
Photonics

Background:

Super-resolution microscopy aims to surpass the diffraction limit for detailed imaging.
Conventional methods involve tip or illumination optimization.
Vector light fields offer novel illumination strategies for enhanced resolution.

Purpose of the Study:

To review recent advances in super-resolution imaging utilizing cylindrical vector beams (CVB).
To summarize the development and strategies of CVB-based super-resolution techniques.
To discuss future directions in CVB-illuminated super-resolution imaging.

Main Methods:

Introduction to the fundamentals and properties of CVB.
Discussion of CVB-based super-resolution imaging strategies (tight focusing, depletion, plasmonic nanofocusing, polarization matching).
Review of CVB illumination techniques in near-field and far-field microscopy.

Main Results:

CVB enables resolution improvement in various imaging modalities like STED and nonlinear imaging.
Summary of CVB-based super-resolution imaging roadmap over the past two decades.
Introduction to tip-scanning, nonlinear, STED, subtraction, and superoscillation imaging using CVB.

Conclusions:

CVB illumination is a powerful tool for advancing super-resolution optical microscopy.
CVB-based techniques offer significant potential for nanomaterial, nanobiology, and nanophotonics research.
Further research is needed to address challenges and explore future directions in CVB-illuminated super-resolution imaging.