Embryonic Development of the Gastrodermis in the Coral Acropora tenuis

Due to limited spawning seasons, embryogenesis of corals has not fully been studied and the embryonic origin of gastrodermis remains uncertain in Acropora. We herein examined how embryonic endodermal cells develop into the gastrodermis and mesentery of polyps in Acropora tenuis. In juvenile polyps, the gastrodermis invaginates to form mesenteries, both of which were stained with rhodamine-phalloidin, an anti-myocyte-specific enhancer factor 2 (anti-AtMef2) antibody, and an anti-lipoxygenase homology domain-containing protein 1 (anti-AtLoxhd1) antibody. Rhoda-mine-phalloidin staining was traced back to the endodermal cells of 60-85 hpf 'pear'-stage embryos through the larval stage. AtMef2 appeared in the blastomeres of a 12-hpf 'prawnchip'-stage embryo that was a variant U-shaped blastula with a narrow blastocoel. AtMef2 temporarily disappeared from the nuclei of 28-hpf 'donut'-stage embryos and reappeared in the endodermal cells of 40-hpf early 'pear'-stage embryos, suggesting a transition from maternal to zygotic expression of Mef2. The blastopore closed without the invagination of blastomeres. The gastrocoel collapsed and the Mef2-positive endoderm was dissociated into single cells in the well-developed blastocoel filled with yolk cells. The mesoglea appeared in the yolk cell layer. AtLoxhd1 was traced back to the endodermal cells of 'pear'-stage embryos. In 11-dpf larvae, Loxhd1-positive endodermal cells elongated in the vicinity of the mesoglea to adhere to each other and form the gastroderm epithelium in larvae. Therefore, in this coral, the inner wall of U-shaped early embryos is the cellular origin of the gastrodermis. Inner wall-derived endodermal cells move independently toward the mesoglea, where cell-cell adhesion occurs to establish the gastrodermis.