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Protocol to culture Leishmania protozoans for lipophosphoglycan extraction and purification

Lisa Ulrike Teufel1, Jéssica Cristina Dos Santos2

  • 1Department of Internal Medicine and Radboudumc Center for Infectious Diseases (RCI), Radboud University Medical Center, Nijmegen, the Netherlands; Department of Medical BioSciences, Research Institute for Medical Innovation, Radboud University Medical Center, Nijmegen, the Netherlands.

STAR Protocols
|December 5, 2024
PubMed

Keywords:
CancerCell BiologyImmunologyMetabolismMicrobiology

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Leishmaniasis is a widespread parasitic disease caused by several Leishmania species. It affects millions of people each year and remains a major public health problem in endemic regions. First-line treatment relies on pentavalent antimonials, including meglumine antimoniate and sodium stibogluconate. Even so, how these drugs work has not been fully clear, especially their interaction with parasite-specific biochemical pathways. One key target is trypanothione reductase (TR), an enzyme that...