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Updated: Jun 5, 2025
10:56
Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
Published on: May 17, 2014
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Intricate ribosome composition and translational reprogramming in epithelial-mesenchymal transition.
Chloé Morin1, Agnès Baudin-Baillieu2, Flora Nguyen Van Long1
1"Ribosome, Translation and Cancer" Team, LabEx DEVweCAN, Institut Convergence Plascan, LYriCAN+, Centre de Recherche en Cancérologie de Lyon, INSERM U1052, CNRS UMR5286, Centre Léon Bérard, Université de Lyon, Université Claude Bernard Lyon 1, Lyon 69008, France.
Summary
Epithelial-mesenchymal transition (EMT) involves changes in cell translation. Ribosomal protein RPL36A levels increase during EMT, driving mesenchymal features and highlighting translation
Area of Science:
- Molecular biology
- Cell biology
- Cancer research
Background:
- Epithelial-mesenchymal transition (EMT) is crucial for development and disease.
- While transcriptional control is studied, translational regulation's role in EMT is emerging.
- Ribosome composition changes may influence cellular phenotypes during EMT.
Purpose of the Study:
- To investigate the role of translational reprogramming and ribosome composition in EMT.
- To identify specific ribosomal components altered during EMT.
- To determine if altered ribosome composition can drive EMT phenotypes.
Main Methods:
- Genome-wide analyses of the translational landscape.
- Riboproteomics to profile ribosome composition.
- Overexpression of specific ribosomal proteins (RPL36A) in cell lines.
Main Results:
- Significant rearrangement of the translational machinery during EMT was observed.
- Overexpression of the EMT transcription factor ZEB1 led to altered translational reprogramming.
- Increased levels of ribosomal protein RPL36A were found in mesenchymal ribosomes.
- RPL36A overexpression alone induced mesenchymal features, including altered morphology and gene expression.
Conclusions:
- Translational reprogramming and ribosome composition are critical regulators of EMT.
- RPL36A is a key driver of EMT, influencing cell morphology and behavior.
- Targeting translation and ribosome composition offers new therapeutic strategies for EMT-related diseases.
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