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Identifying immune cell infiltration and effective diagnostic biomarkers for ischemic stroke using bioinformatics
Zongyong Zhang1,2,3, Zongqing Zheng1,2,3, Wenwei Luo4
1Department of Neurosurgery, The First Affiliated Hospital, Fujian Medical University, Fuzhou, China.
Plos One
|December 5, 2024
Summary
This study identifies key ubiquitination-related genes and immune cell changes associated with ischemic stroke (IS). These findings offer potential biomarkers for early diagnosis and therapeutic targets in IS patients.
Area of Science:
- Biomedical research
- Genomics
- Immunology
Background:
- Ischemic stroke (IS) is a major global cause of death and disability.
- Early diagnosis and identification of marker genes are critical for improving IS outcomes.
- Understanding the molecular mechanisms underlying IS is essential for developing effective treatments.
Purpose of the Study:
- To identify ubiquitination-related differentially expressed genes (DEGs) in ischemic stroke.
- To explore the correlation between gene expression, signaling pathways, and immune cell infiltration in IS.
- To uncover potential diagnostic biomarkers and therapeutic targets for IS.
Main Methods:
- Utilized weighted gene co-expression network analysis (WGCNA) on public gene expression datasets (GSE22255, GSE37587).
- Applied differential gene expression analysis (limma) and gene set enrichment analysis (GSEA, GSVA).
- Constructed interaction networks (RBP-mRNA, mRNA-miRNA-lncRNA, PPI) and performed receiver operating characteristic analysis.
Main Results:
- Identified 33 ubiquitination-related DEGs and 16 hub genes in IS.
- Found significant differences in immune cell infiltration, with higher levels of specific T helper cells, eosinophils, and mast cells in IS patients.
- Established correlations between gene expression (DHFR2, DNAAF2) and immune cell infiltration.
Conclusions:
- Identified key DEGs, hub genes, enriched pathways, and immune cell signatures involved in IS.
- These findings highlight potential diagnostic markers and therapeutic targets for ischemic stroke.
- Further research is warranted to elucidate the functional roles of identified genes in IS pathogenesis.
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