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Highly responsive cryogel based sensing platform by encapsulating programmed DNA for colorimetric detection of
Yujia Guo1, Jiaxuan Xiao1, Fengxiang Ai1
1School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, China.
Analytica Chimica Acta
|December 5, 2024
Summary
Responsive cryogels enable rapid detection of 17β-estradiol (E2). This novel hydrogel biosensor integrates with smartphones for sensitive, portable analysis, overcoming traditional limitations.
Area of Science:
- Materials Science
- Analytical Chemistry
- Biotechnology
Background:
- Responsive hydrogels offer advantages in detection due to their unique network and optical properties.
- Slow response times of conventional hydrogels hinder their application, necessitating improved responsiveness.
Purpose of the Study:
- To design and fabricate a highly responsive cryogel for the rapid analysis of 17β-estradiol (E2).
- To develop a portable colorimetric sensing platform for sensitive E2 detection.
Main Methods:
- Hierarchical cryogels were engineered by manipulating hydrogel structure via a freezing process.
- DNA hairpins within the cryogel facilitated rapid adsorption of complementary DNA and activated hybridization chain reaction (HCR) in the presence of E2.
- Integration with a smartphone system created a portable colorimetric sensing platform.
Main Results:
- The cryogel exhibited enhanced responsive properties.
- A sensitive detection of E2 was achieved with a low detection limit of 0.5 nM.
- The developed platform demonstrated rapid and portable target detection capabilities.
Conclusions:
- This study presents a promising approach for designing highly responsive hydrogel-based biosensors.
- The developed cryogel-based platform broadens the application of hydrogels in rapid and portable detection systems.
