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CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy Applications

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Clotted Apheresis Hematopoietic Stem Cell Product.

Lefan Zhuang¹, Jennifer Woo², Franchesca Francisco¹

¹Division of Transfusion Medicine, Department of Pathology, City of Hope National Medical Center, Duarte, California, USA.

Journal of Clinical Apheresis

|December 6, 2024

Summary

Fresh hematopoietic progenitor cell (HPC) products can unexpectedly clot after collection. This case highlights the potential for rapid clot formation in unprocessed HPC collections, suggesting the need for anticoagulants.

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Expansion and Enrichment of Gamma-Delta γδ T Cells from Apheresed Human Product

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Published on: September 22, 2021

Area of Science:

Hematology
Transfusion Medicine
Cellular Therapy

Background:

Hematopoietic progenitor cells (HPC) are typically collected via leukapheresis.
Anticoagulants are routinely used during leukapheresis to prevent circuit and product clotting.
Clotting of fresh HPC product post-collection is not a reported complication.

Observation:

A case of progressive clotting in a fresh, unprocessed HPC product from a matched unrelated donor is presented.
Initial observation revealed thin, thread-like white clots within the HPC bag.
Aseptic aliquoting led to rapid clot coalescence into a large, jelly-like mass within 60 minutes.

Findings:

The fresh HPC product exhibited spontaneous and rapid clot formation.
The clotting process was progressive, transforming small clots into a significant mass.
This observation challenges the assumption that fresh HPC products are immune to clotting before processing.

Implications:

This case suggests that fresh HPC products may be susceptible to clotting even without cryopreservation.
The findings indicate a potential need for anticoagulant addition to fresh HPC products before manipulation.
Further investigation into the mechanisms of spontaneous clotting in HPC products is warranted to ensure product viability and safety.