Low-cost automated cell counting module fabricated using CNC milling and soft lithography
Takanobu Takenouchi1,2, Yuta Iijima3, Kazuyo Ito2,4
1Department of Applied Physics, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
Hardwarex
|December 6, 2024
Summary
Researchers developed a low-cost, compact cell counting module for cell biology and bioengineering. This affordable device offers reasonable accuracy and throughput, serving as a practical alternative to expensive systems.
Area of Science:
- Biotechnology
- Bioengineering
- Cell Biology
Background:
- Cell counting is fundamental in biological research.
- Existing cell counting systems vary in performance, cost, and size.
- There is a need for accessible and efficient cell counting solutions.
Purpose of the Study:
- To develop a cost-effective, scalable, and compact cell counting module.
- To create an automated cell counting system utilizing the developed module.
- To evaluate the module's accuracy, throughput, and sensitivity for cellular experiments.
Main Methods:
- Designed and assembled a compact cell counting module (29 × 48 × 16 mm, $165 USD).
- Utilized commercially available optical and electronic components.
- Employed CNC milling and soft lithography for housing fabrication.
- Integrated the module into an automated system using open-source platforms.
Main Results:
- The module demonstrated reasonable accuracy (0-500 cells/µL) and sorting resolution (5 µm and 15 µm particles).
- Achieved a low cost of $165 USD per module.
- The compact size and ease of assembly were highlighted.
Conclusions:
- The developed cell counting module is a viable, low-cost alternative for routine cell experiments.
- This technology can benefit researchers in cell biology and bioengineering.
- The module's scalability and affordability enhance accessibility in scientific research.
