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Updated: Jun 5, 2025

Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples
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Cofactor F420 tail length distribution in different environmental samples.

Mathias Wunderer1, Rudolf Markt1, Eva Maria Prem1

  • 1Universität Innsbruck, Department of Microbiology, Technikerstrasse 25d, 6020, Innsbruck, Austria.

Heliyon
|December 6, 2024
PubMed
Summary

Cofactor F420, an essential electron carrier, is abundant in manure and digester sludge, highlighting its vital role in both anaerobic and aerobic microbial metabolism. This cofactor is crucial for redox reactions in prokaryotes.

Keywords:
Environmental habitatF420 producing microorganismMicrobial community

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Area of Science:

  • Microbiology
  • Biochemistry

Background:

  • Cofactor F420 is a vital electron carrier in microbial redox reactions.
  • Its low redox potential makes it crucial for primary and secondary metabolism.
  • Increasing interest in F420's role in diverse microbial communities.

Purpose of the Study:

  • Investigate cofactor F420 glutamyl tail length spectra in various habitats.
  • Analyze microbial communities associated with F420 production.
  • Identify F420-producing microorganisms using amplicon sequencing and in-silico methods.

Main Methods:

  • High-performance liquid chromatography (HPLC) for F420 quantification.
  • Amplicon sequencing for microbial community analysis.
  • In-silico analysis to identify F420-producing microbes.

Main Results:

  • Highest F420 concentrations found in horse manure, digester sludge, and mixed manure (approx. 100-fold higher).
  • Significant F420 presence in samples with high oxygen availability, indicating a role in aerobic metabolism.
  • F420-3 was the most abundant derivative in most samples, crucial for prokaryotic redox reactions.

Conclusions:

  • Cofactor F420 is ubiquitous and plays a critical role in both anaerobic and aerobic microbial metabolism.
  • F420-3 is a key derivative involved in primary and secondary metabolic redox reactions across prokaryotes.
  • Manure and digester sludge are rich sources of cofactor F420.