Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nucleic Acid Structure01:25

Nucleic Acid Structure

5.9K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
5.9K
RNA Structure01:19

RNA Structure

4.7K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
4.7K
Types of RNA01:20

Types of RNA

5.6K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
5.6K
Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K
Nucleic Acids02:43

Nucleic Acids

43.7K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
43.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exercise Capacity, Endothelial Function, Muscle Mass, and Strength in Pediatric Patients With Fontan Circulation.

CJC pediatric and congenital heart disease·2026
Same author

Early Referral of Individuals with Fontan Physiology to Heart Failure Specialists: A Fontan Action Plan.

Pediatric cardiology·2026
Same author

The Evolving Epidemiology of Invasive Pneumococcal Disease in Australian Children: A Multicentre Retrospective Observational Study.

Open forum infectious diseases·2026
Same author

The congenital heart early-career exchange programme: collaboration to advance faculty development.

Cardiology in the young·2026
Same author

A polymerase ribozyme increases copying fidelity through pyrophosphate-mediated RNA repair.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

A Phase 2, Open-Label, Multicenter Study of the Safety and Efficacy of TAK-007 in Adult Patients with Relapsed/Refractory B-cell Non-Hodgkin Lymphoma.

Blood cancer discovery·2026

Related Experiment Video

Updated: Jun 5, 2025

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
11:37

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

18.9K

Bioorthogonal Cyclopropenones for Investigating RNA Structure.

Sharon Chen, Christopher D Sibley1, Brandon Latifi

  • 1Chemical Biology Laboratory, National Cancer Institute, Frederick, Maryland 21702, United States.

ACS Chemical Biology
|December 6, 2024
PubMed
Summary

Researchers developed a new chemical method to study RNA structures in cells. This bioorthogonal cyclopropenone (CpO) approach allows for precise RNA cross-linking, offering a clearer view of RNA's role in cellular processes and disease.

More Related Videos

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

4.0K
Monitoring Equilibrium Changes in RNA Structure by 'Peroxidative' and 'Oxidative' Hydroxyl Radical Footprinting
13:41

Monitoring Equilibrium Changes in RNA Structure by 'Peroxidative' and 'Oxidative' Hydroxyl Radical Footprinting

Published on: October 17, 2011

14.2K

Related Experiment Videos

Last Updated: Jun 5, 2025

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
11:37

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

18.9K
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

4.0K
Monitoring Equilibrium Changes in RNA Structure by 'Peroxidative' and 'Oxidative' Hydroxyl Radical Footprinting
13:41

Monitoring Equilibrium Changes in RNA Structure by 'Peroxidative' and 'Oxidative' Hydroxyl Radical Footprinting

Published on: October 17, 2011

14.2K

Area of Science:

  • Molecular Biology
  • Chemical Biology
  • Biochemistry

Background:

  • RNA structure is crucial for protein production and cellular functions.
  • Misfolded RNAs are implicated in various diseases, but their structures are not fully understood.
  • Current methods for studying RNA structures in native environments have limitations, including high background noise.

Purpose of the Study:

  • To develop a novel, chemically triggered method for interrogating RNA structures in native cellular environments.
  • To establish more accurate RNA structure-function relationships.
  • To overcome the limitations of existing RNA probing tools.

Main Methods:

  • Development of bioorthogonal cyclopropenones (CpOs) as chemical triggers for RNA cross-linking.
  • Conjugation of a CpO motif to thiazole orange (TO-1) to create TO-1-CpO probe.
  • Application of TO-1-CpO to a model RNA aptamer (Mango) and subsequent chemical triggering with phosphines to induce cross-linking.

Main Results:

  • TO-1-CpO demonstrated selective binding to the Mango RNA aptamer with nanomolar affinity, indicated by fluorescence.
  • Chemical triggering with phosphines successfully induced covalent cross-linking between the CpO and RNA.
  • Cross-linking efficiency was shown to be dependent on both time and reagent dose.

Conclusions:

  • The study presents a novel, chemically triggered approach for RNA cross-linking using bioorthogonal cyclopropenones.
  • This method provides a valuable new tool for studying RNA structures and conformations in native biological settings.
  • The developed probes expand the available toolkit for RNA research, potentially advancing our understanding of RNA function and disease.